(23) Sorbitol Transport, Sorbitol, and Watercore in Apple Cultivars Differ in Watercore Susceptibility

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Sorbitol metabolism and sink-source interconversions in developing apple leaves.

In apple (Malus domestica Borkh.) sorbitol is the primary product of photosynthesis, the major translocated form of carbon, and a common fruit constituent and storage compound. Previous work on sorbitol metabolism has revealed a NADPH-dependent aldose 6-phosphate reductase (A6PR) in green tissues, and a NAD-dependent sorbitol dehydrogenase in nongreen tissues. Results here show a decrease in so...

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Detection and characterization of sorbitol dehydrogenase from apple callus tissue.

Sorbitol dehydrogenase (l-iditol:NAD(+) oxidoreductase, EC 1.1.1.14) has been detected and characterized from apple (Malus domestica cv. Granny Smith) mesocarp tissue cultures. The enzyme oxidized sorbitol, xylitol, l-arabitol, ribitol, and l-threitol in the presence of NAD. NADP could not replace NAD. Mannitol was slightly oxidized (8% of sorbitol). Other polyols that did not serve as substrat...

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Watercore in Apples: Causes, concerns, detection and sorting

Watercore is a serious physiological disorder of apples that occurs on the tree. It is typified by water-soaked areas of the cortex, which cause the tissue to become translucent. In its mildest cases, watercore is localized near the primary vascular tissue running through the cortex (Fig. 1, upper left). The disorder is highly cultivar-dependent; however, watercore has been reported in most cul...

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Sorbitol dehydrogenases in Acetobacter suboxydans.

It has previously been shown that cell-free extracts of Acetobacter subozydans possess two pathways for the oxidation of sorbitol (1). In the presence of DPN,l fructose is produced; with TPN, sorbose is formed. The primary purpose of this paper is to describe the separation of these two dehydrogenases from each other. The DPN enzyme free from TPN activity has been concentrated about lBfold, whe...

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Ubiquitous distribution and different subcellular localization of sorbitol dehydrogenase in fruit and leaf of apple

NAD(+)-dependent sorbitol dehydrogenase (NAD-SDH, EC 1.1.1.14), a key enzyme in sorbitol metabolism, plays an important role in regulating sink strength and determining the quality of apple fruit. Understanding the tissue and subcellular localization of NAD-SDH is helpful for understanding sorbitol metabolism in the apple. In this study, two NAD-SDH cDNA sequences were isolated from apple fruit...

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ژورنال

عنوان ژورنال: HortScience

سال: 2005

ISSN: 0018-5345,2327-9834

DOI: 10.21273/hortsci.40.4.993d